Luminex xPONENT for MAGPIX User Manual
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RP1
Refers to the dyes bound to the surface of the xMAP microsphere. Also
see reporter channel.
sample
The mixture of assay components (microspheres, reporter, patient
dilutant) that is analyzed.
sample probe
The probe, or needle, on the Luminex analyzer that acquires sample
from the 96-well plate.
sample reaction
The reaction that occurs between reagents and beads.
signal
Detectable measurement unit of the reporter molecule.
standards microspheres,
assay
Assay standards are substances of known concentrations used to derive
a standard curve with which unknown samples and controls are
compared to determine their concentration or quantity. See control
microspheres, assay.
suspension
Solution consisting of homogeneously dispersed microspheres in an
aqueous medium.
system controls
Include the MagPlex xMAP reporter and classification control
microspheres. They are used to verify the calibration of the Luminex
analyzer.
test
Each test represents an analyte and corresponds to a bead set. Each
bead set falls into a particular location on the bead map.
verifier microspheres,
assay
Used to verify standards within the kit. Tells you that the curve or
thresholds are correct.
verifier microspheres,
system
xMAP microspheres used to verify the calibration and optical integrity for
the Luminex analyzer.
xMAP
See Luminex xMAP microsphere set.
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