Bio-Rad Microplate Manager Software - Manual (Page 77)

Chapter 5. Protocols
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After you have defined your plate types and other configurations in the Twister
control panel, select the appropriate plate type from the pull-down menu under Plate
Type in the Plate Handler Options dialog box.
The Restacking Option buttons allow you to specify whether and how you will
restack your plates. Restack at End of Rack will restack the plates after each rack is
emptied. Restack at End of Batch will wait to restack the plates until all the plates in
all the racks have been read.
If you have a rack for storing lids on your Twister (specified in the Twister control
panel), you can select the appropriate option in the Lid Configuration field.
If the Twister Extended Capacity platform has been installed, you can select the Use
Extended Capacity checkbox. If you do not want to use the Extended Capacity,
deselect this checkbox.
If a barcode reader is installed with your Twister, the Scan Barcode checkbox will
appear checked. If you do not want to scan barcodes, deselect this checkbox. If a
plate has a barcode, it will be stored in the plate file and printed on all the reports. (It
will not be visible in the reports displayed on the screen.) If a plate has no barcode,
the barcode reader will return a blank string.
Number of Plates
If the Stack Loader checkbox is selected in the main protocol window, the Num of
Plates field will be grayed out. As the stack loader progresses through a stack of
plates, the Num of Plates Read field will indicate the number of plates that have been
read.
If you want to load your plates by hand, deselect the Stack Loader checkbox and
enter the number of plates in the Num of Plates field. Then, when you click on Run,
you will be prompted to insert the next plate in the sequence after each plate has been
read.
Filename Field
In the course of a batch of multiple plate readings, each data set is saved as soon as
each reading is complete. Before you run a multiple plate protocol, you must specify