Bio-Rad Microplate Manager Software - Manual (Page 61)

Chapter 5. Protocols
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5.1 Features Common to All Protocols
All three protocol types share basic reading parameters such as measurement
wavelength, reader type, and delay; basic plate template and analysis parameters;
reporting features; and functions for saving the protocol and running the reader.
5.1.1 Reader Identification
When you select a new or existing protocol from the FILE menu, the software first
scans for the SCSI or serial port connection to the microplate reader. (For
instructions on specifying a port, see section 2.5.)
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If no reader is found, you will receive a warning message. Make sure the reader
is turned on, and check all the connections to the reader. See the microplate
reader hardware manual for more details.
5.1.2 Selecting a Template
Each protocol file includes a microplate template that specifies the number of wells
to be read, as well as any additional formatting information you want to include
(locations of standards, unknowns, controls, etc.). This template is copied into the
data file when a reading is taken.
From within the protocol dialog box, you can either create a new template (Ultramark
only) or pick an existing template. See Chapter 4 for a description of templates and
instructions on creating and saving them.
If no template is selected, a default template of 96 blank wells (12 x 8) is used.
Selecting an Existing Template
To select an existing template for your plate reading, click on the Pick Template...
button. This will open a File Open dialog box, from which you can select the
predefined template to include in the protocol file.
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Templates cannot be copied from existing protocol files. They must be copied
from data files (.mpm extension).