Bio-Rad Microplate Manager Software - Manual (Page 30)

Microplate Manager User Guide
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Scenario 2. Kinetic Assay
You want to perform a new kinetic assay. From the FILE menu, you select New
Kinetic Protocol. The Kinetic Protocol dialog box opens.
For this assay, you would like to make five readings at 21-second intervals followed
by 20 readings at one-minute intervals. You want to shake the plate for one second
before each of the first five readings.
To accomplish this, you define two reading sets. The first set has 5 repetitions, a 1-
second mix time, and a 22-second interval (the interval you want plus the mix time).
The second set has 20 repetitions, 0 seconds mix time, and a 60-second interval.
Figure 11. Defining the Reading Sets.
After inserting your 12 x 8 plate into the reader, you click on Run and the reader
begins collecting data. At this point, both the template and the Kinetic Plots Report
automatically pop up.
The Kinetic Plots Report shows a graph of absorbance versus time for each well. As
each reading is completed, the graphs are updated. After all of the data has been read,
you can display any relevant report (Limit, Control, etc.) by selecting it from the
VIEW menu. To change the velocity calculation, you go to Analysis Options... under
the OPTIONS menu and select one of the two calculation methods under Kinetic
Mode. When you click on OK, the velocities are automatically recalculated.
Interval includes
mix time