Bio-Rad Microplate Manager Software - Manual (Page 69)

Chapter 5. Protocols
59
Absorbance Delay
Absorbance Delay delays data collection until a well that you select reaches a
specified level of absorbance. To access the controls for this feature, click on the
Absorbance Delay button.
Figure 42. Absorbance Delay dialog box.
In the Absorbance Delay dialog box, click on the Use Absorbance Delay checkbox
to enable this feature. Select the row and column number of the well whose
absorbance you want to monitor from the pull-down lists. In the Acquire when the
reading is section, specify an absorbance that is above or below a certain OD value.
Below will begin data collection when the measured absorbance is below the
specified OD, and Above will begin data collection when the absorbance is equal to
or greater than the specified OD.
Click on the OK button to implement your changes.
5.3 Features Unique to Kinetic Protocols
Kinetic protocols are used to acquire a series of absorbance readings from each well
over a time period that you specify. You can use these protocols to calculate reaction
velocities.