Bio-Rad Microplate Manager Software - Manual (Page 100)

Microplate Manager User Guide
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Figure 65. Limits dialog box.
Limits are assigned independently for each assay in a protocol. When there is more
than one assay, tabs labeled with each assay number appear in the dialog box. To
switch from one assay to another, click on the appropriate tab.
To set your own limits, select the Use Custom Limits option and enter numbers in the
appropriate fields.
½
For kinetic data, your limits will be adjusted velocities; for endpoint data, your
limits will be absorbances.
If Use Default Limits is selected, the unknown sample with the greatest absorbance
(for kinetic reports, the sample with the greatest adjusted velocity) will be used as the
upper limit, and the unknown sample with the lowest absorbance (lowest adjusted
velocity) will be used as the lower limit. If no unknowns are specified, the default
value for both upper and lower limits is 0.000.