Bio-Rad Microplate Manager Software - Manual (Page 71)

Chapter 5. Protocols
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To activate a reading set, click on the checkbox next to the set number. Only sets that
are checked will be run.
Enter the number of readings you want to take in the Readings column.
Next enter the interval between the start of two consecutive readings in the Interval
column. Note that this is the minimum time necessary for one complete reading plus
any mixing time you specify. For a standard 12
x 8 (96-well) plate, the smallest
interval you can specify is 7 seconds. For custom plate sizes, the smallest interval you
can specify is 450 seconds (7.5 minutes). If the interval you enter is too short, a
message box will tell you the minimum total read time.
Finally, you can specify a mixing time for your plate. If no mixing is desired, enter a
0.
½
If you specify a mixing time, be sure to include this time in the interval between
readings (Interval column).
5.3.2 Analysis Options
To access the other kinetic protocol options, click on the Analysis Options... button.
This will open the Analysis Options dialog box.