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Bio-Rad Microplate Manager Software User Manual

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Chapter 3. Getting Started

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Figure 10. Performing an endpoint reading.

You have a data file saved from a previous run with the template you want to use for
this plate, so you click on Pick Template... and choose that data file. Now that
everything is set, you click on the Run button.

After the reading is complete, the raw data values pop up. The plate template is also
displayed. Since the template already contains the concentrations of the standards,
you go straight to the Unknown Concentration Report (select Unknown
Concentration Report
from the VIEW menu). You select Print... from the FILE
menu to print this report.

You decide that you will be repeating this operation frequently, so you go back to the
Endpoint Protocol dialog box. The Reports... button opens a dialog box in which
you specify the reports to view and/or print after each reading. Select the Unknown
Concentration Report
for printing, and click on OK. You select Save Protocol from
the FILE menu and enter a file name to store this protocol on disk.

The next day, you want to repeat the protocol. Select the protocol file name from the
Open dialog box, place your plate in the reader, click on the Run button, and wait at
the printer for a hard copy of the new Unknown Concentration Report.