Bio-Rad Microplate Manager Software - Manual (Page 59)

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5. Protocols
Protocols contain the settings for controlling the microplate reader. To read a
microplate, you must first open a protocol and specify the settings.
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Some of the features and functions in the protocols are different depending on
the type of microplate reader (Ultramark, Model 550, Benchmark) you are using.
These differences are noted in the text. The figures depict some functions that
are only available with the Ultramark.
Microplate Manager has three general types of protocols: Endpoint, kinetic, and
multiple plate.
•
Endpoint protocols are used to acquire a single absorbance reading from each
well.
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Kinetic protocols are used to acquire a series of absorbance readings from each
well over a user-defined interval. You can use these protocols to calculate
reaction velocities.
•
Multiple plate protocols are used with a stack loader to collect endpoint data
from a series of plates. Multiple plate protocols save each successive endpoint
data set in a separate file.
First you select the type of protocol you want to use, then you specify the settings for
your particular reading, and finally you take the reading. If you want to reuse the
particular protocol you’ve created, you can save it as a file.
Creating a New Protocol
To create a new protocol, select New Kinetic Protocol, New Endpoint Protocol, or
New Multiple Plate Protocol from the FILE menu. This will open the dialog box for
that particular type of protocol.
Opening an Existing Protocol
To edit an existing protocol file, choose Open... from the FILE menu, and then select
the protocol name from the Open File dialog box. File names for protocols have the