2 kit components, 3 storage conditions – Bio-Rad Experion RNA Analysis Kits User Manual
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Table 1.1. Experion RNA analysis kit specifications.
Experion RNA StdSens
Experion RNA HighSens
Number of samples
12
11
Sample volume
1 µl
1 µl
Quantitation range:
Total RNA
25–500 ng/µl
200–5,000 pg/µl
mRNA
25–250 ng/µl
—
Limit of detection (total RNA)
5 ng/µl
100 pg/µl
Maximum salt concentration
TE buffer (10 mM Tris, 1 mM EDTA)
DEPC-treated water
1.2 Kit Components
Table 1.2. Components of the Experion RNA analysis kits.
Item
Description
Volume (per Vial)
10-Chip Kit
RNA StdSens or
Microfluidic chips used for RNA separation
10 chips
RNA HighSens chip
Cleaning chip
Chip used for cleaning electrodes
2 chips
RNA gel
Proprietary polymeric sieving matrix
1,250 µl
1 vial
RNA StdSens or
Proprietary fluorescent dye
20 µl
1 vial
RNA HighSens stain
RNA StdSens or
Buffer for sample preparation; contains lower marker
900 µl
1 vial
RNA HighSens loading buffer
for alignment of samples to the RNA ladder
RNA ladder
Standard containing 8 RNA fragments of 200–6,000 nt
20 µl
1 vial
RNA sensitivity enhancer
Proprietary reagent to improve sensitivity
100 µl
1 vial
(RNA HighSens kit only)
Spin filters
Used for filtering reagents during sample preparation
3 filters
1.3 Storage Conditions
Table 1.3. Storage conditions.
Item
Storage
Shelf Life
Experion reagents
4ºC
See expiration date on packaging
Experion chips
Ambient
See expiration date on packaging
Experion RNA ladder
–70ºC
See expiration date on packaging
Filtered gel (G)
4ºC
1 month from filtration; after 1 month, refilter
Experion RNA StdSens and HighSens Analysis Kits