Bio-Rad Experion RNA Analysis Kits - Manual (Page 19)

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Do not open the lid of the Experion electrophoresis station until the run is complete. The lid does
not lock. Opening the lid aborts the run.
An “IV Check Error” message indicates the system cannot make electrical contact in one of the
wells. This often means there is a bubble at the bottom of the well. Abort the run, and check the
chip for bubbles or empty wells. Refill the affected well(s), and start the run again.
6. Click Start. The green LED in the center of the front panel on the electrophoresis station blinks, and
the system performs a number of checks: it confirms that a chip has been inserted, that all wells
contain liquid, that electrical connections are made, etc. A calibration counter marks the progress of
these calibrations at the upper right of the screen.
Never store the cleaning chip inside the electrophoresis station. Store the empty cleaning chip
covered to keep the wells clean. Two cleaning chips are included with each box of chips.
7. During separation, the sample name is highlighted in the project tree and the electropherogram
trace, and virtual gel bands appear in real time:
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The electropherogram of the sample being separated appears in the electropherogram
view
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The lane corresponding to that sample is outlined in pink and has a dark background
To display the electropherogram of another sample, click on either the sample name in the project
tree or on a lane in the virtual gel.
8. When analysis is complete (after ~30 min), the instrument beeps and a window opens indicating the
end of the run. Select OK and remove the chip from the chip platform.
9. Clean the electrodes using deionized water within 30 min of each run.
3.8 Clean the Electrodes
1. Fill a cleaning chip with 800 µl deionized water (0.2 µm-filtered). Gently tap the side of the cleaning
chip to remove any trapped bubbles from the wells.
2. Place the cleaning chip on the chip platform in the electrophoresis station, close the lid, and leave it
closed for 1 min.
3. Open the lid, remove the cleaning chip, and allow the electrodes to dry for 1 min. Close the lid.
4. Replace the water in the cleaning chip after use to avoid contamination. For storage, remove the
water from the cleaning chip and store the chip in a clean location.
3.9 Evaluate the Run
When a run is complete, evaluate the run and the analysis of the data by Experion software.
1. Ensure that all lanes (ladder and samples) are visible in the virtual gel. The lower marker (indicated
by a pink triangle) should be visible in and aligned across all lanes. If the marker peak is not properly
assigned, you may need to manually set the marker (see Section 6.1).
Experion RNA StdSens and HighSens Analysis Kits