Bio-Rad Experion RNA Analysis Kits - Manual (Page 6)

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1.1 Product Description
The Experion RNA StdSens and HighSens analysis kits are used for RNA analysis with the Experion
automated electrophoresis system (Figure 1.1). The Experion system employs LabChip microfluidic
technology to automate nucleic acid and protein electrophoresis and analysis, integrating separation,
detection, and data analysis within a single platform. Using much smaller sample and reagent quantities
than standard analysis methods, the Experion automated electrophoresis system can be used both
upstream and downstream of a number of nucleic acid and protein applications.
The Experion RNA analysis kits are used to determine total RNA and mRNA integrity, purity, and
concentration. The Experion RNA StdSens analysis kit offers analysis at nanogram levels, and the
Experion RNA HighSens analysis kit is used for analysis at picogram levels (Table 1.1). Both kits include
the Experion RNA ladder, which has been optimized for automated electrophoresis on the Experion
system. They also feature the Experion RNA loading buffer, which contains a 50 bp marker that is used
for the proper alignment of samples to the RNA ladder.
RNA analysis with the Experion system compares favorably with other methods for RNA quantitation,
such as UV spectroscopy, and provides analysis of both RNA integrity and concentration in a format
that is versatile and easy to analyze. In addition, Experion analysis is relatively unaffected by reagents
common to RNA preparation, and the electropherogram it generates enables evaluation of the RNA
sample for degradation and for the presence of copurifying nucleic acids, such as genomic DNA and
tRNA (Woo and Strong 2006). The Experion RNA StdSens and HighSens analysis kits offer single-step
total RNA and mRNA analysis in less than 30 min.
For details about how the Experion RNA analysis kits analyze RNA, refer to Appendix A in this manual.
Fig . 1 .1 . The Experion system . The system
includes the following components: 1) automated
electrophoresis station, 2) priming station,
3) vortex station used for nucleic acid analysis
only, 4) system operation and data analysis tools
(software), and 5) analysis kits, which include the
(a) chips and (b) reagents for protein (Pro260 kit),
standard-sensitivity RNA (StdSens kit),
high-sensitivity RNA (HighSens kit), and DNA
(DNA 1K and 12K kits) analyses.
Register your Experion system to ensure you receive important updates on software, tech notes,
and manuals. Upon installation, a dialog provides registration instructions.
Experion Automated Electrophoresis System
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5a
5b