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Bio-Rad Experion RNA Analysis Kits User Manual

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RNA ladder
RNA standard containing 8 RNA fragments of
200–6,000 nt; separation data from the ladder are
used by Experion software for size determination
of sample fragments and quantitation of RNA
samples

RNA quality indicator (RQI)
Quantitative measure of integrity of eukaryotic
total RNA, generated by Experion software and
ranging from 1 (completely degraded) to 10 (intact)

Run file
File containing data for a single run

Single-well view

Function of Experion software that allows an
electropherogram from a single well to be viewed

Sizing
Determination of the size, in nt, of an RNA
fragment

Spin filter

Polypropylene spin tubes with filter membranes
used to remove particulate matter from the
gel-stain (GS) and gel (G) by centrifugation

Tree view

Portion of the Experion software user interface
dedicated to display of the project tree; used to
locate and manipulate data files and organize runs
into projects

Virtual gel

Densitometric, gel-like image generated from
electropherogram data and displaying peaks as
bands; displayed in the gel view

Vortex station
Instrument used to mix RNA or DNA samples and
loading buffers (supplied in the kits) in the chip
wells; used for nucleic acid applications only

Experion RNA StdSens and HighSens Analysis Kits