Bio-Rad Experion RNA Analysis Kits - Manual (Page 41)

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6.3 Changing General Settings
These settings appear in the Settings tab, in the All Wells Settings dialog under General Settings
(Figure 6.2). They apply to all samples in a run.
n
Data Frequency — displays the rate of data collection in Hz; cannot be edited
n
Filter Width — determines the width of the polynomial (in sec) to be applied to the data for
filtering (noise reduction). Set this variable to less than twice the width of peaks (0.3–10)
n
Polynomial Order — defines the power series applied to fit the raw data. Valid values are
1–10, with lower values yielding smoother curves
Fig . 6 .2 . General Settings options .
6.4 Baseline Modification
Experion software subtracts the average baseline value from the signal. This does not affect the shape
of the curve; it shifts the baseline down to zero. The default settings for baseline modification are
appropriate for most samples.
To shut off this baseline modification method for a selected sample:
1. Under the Settings tab, deselect the Zero Baseline option under Baseline Correction.
2. Click Apply to apply the changes and reanalyze the data, and click Save in the main screen toolbar
to save the new conditions or choose Reset to Default and Apply to recover the default settings.
To edit the parameters used by Experion software for adjusting the baseline for all samples:
1. Either open the Settings tab or select Analysis > All Wells Settings.
2. In the All Well Settings dialog, expand Algorithm Inclusion Settings and deselect the Zero
Baseline modification. Click OK.
3. In the Experion Application dialog, click Yes to save changes and reanalyze the data. Click Save in
the main screen toolbar to save the new conditions.
To reset to default settings, click Reset to Default in the Settings tab under All Well Settings and then
click Save Run in the main screen toolbar.
Experion RNA StdSens and HighSens Analysis Kits