Bio-Rad Experion RNA Analysis Kits - Manual (Page 36)

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5.1 RNA Quantitation
Experion ™ software begins RNA quantitation by comparing the time-corrected area (corrected area)
under the electropherogram of a sample (excluding the lower marker) with the corrected area under the
electropherogram of the RNA ladder. It then uses this value, the ladder concentration (160 ng/μl for the
RNA StdSens assay and 1,000 pg/μl for the RNA HighSens assay), and a correction factor to determine
the RNA concentration of the sample. Results are displayed under the Results and Run Summary tabs
(Figure 5.1).
5.2 RNA Quality Indicator (RQI)
The RNA quality indicator (RQI) feature of Experion software allows you to estimate the level of
degradation in a eukaryotic total RNA sample. The RQI algorithm compares the electropherogram of
RNA samples to data from a series of standardized, degraded RNA samples and automatically returns
a number between 10 (intact RNA) and 1 (degraded RNA) for each sample (Table 5.1). RQI analysis is
performed for all samples with an Experion StdSens or HighSens analysis kit.
To view RQI data, select either the Results (selected sample) or Run Summary (all samples) tab
(Figure 5.1).
Fig . 5 .1 . Experion quantitation results . RQI results appear with quantitation results under the Results (top)
and Results Summary (bottom) tabs. Examples shown are for a total RNA Experion RNA HighSens analysis.
Experion Automated Electrophoresis System