Bio-Rad GS-900™ Calibrated Densitometer - Manual (Page 68)

132 133
2-D Electrophoresis Guide Appendices
Toth GB and Pavia H (2001). Removal of dissolved brown algal
phlorotannins using insoluble polyvinylpolypyrrolidone (PVPP).
J Chem Ecol 27, 1899–1910.
Tu C et al. (2010). Depletion of abundant plasma proteins and
limitations of plasma proteomics. J Proteome Res 9, 4982–4991.
Van den Bergh G and Arckens L (2008). Reducing sample complexity
by RP-HPLC: beyond the tip of the protein expression iceberg.
Methods Mol Biol 424, 147–156.
Wang W et al. (2008). Optimizing protein extraction from plant tissues
for enhanced proteomics analysis. J Sep Sci 31, 2032–2039.
Weiss W et al. (1992). Application of sequential extraction procedures
and glycoprotein blotting for the characterization of the 2-D
polypeptide patterns of barley seed proteins. Electrophoresis 13,
770–773.
Wessel D and Flugge UI (1984). A method for the quantitative recovery
of protein in dilute solution in the presence of detergents and lipids.
Anal Biochem 138, 141–143.
Westermeier R and Scheibe B (2008). Difference gel electrophoresis
based on lys/cys tagging. Methods Mol Biol 424, 73–85.
Wilkins MR et al. (1996). Progress with proteome projects: why all
proteins expressed by a genome should be identified and how to do it.
Biotechnol Genet Eng Rev 13, 19–50.
Xixi E et al. (2006). Proteomic analysis of the mouse brain
following protein enrichment by preparative electrophoresis.
Electrophoresis 27, 1424–1431.
Yuan X and Desiderio DM (2005). Proteomics analysis of
prefractionated human lumbar cerebrospinal fluid. Proteomics 5,
541–550.
Zerefos PG et al. (2006). Characterization of the human urine
proteome by preparative electrophoresis in combination with 2-DE.
Proteomics 6, 4346–4355.
Zischka H et al. (2003). Improved proteome analysis of
Saccharomyces cerevisiae mitochondria by free-flow electrophoresis.
Proteomics 3, 906–916.
Zuo X and Speicher DW (2000). A method for global analysis of
complex proteomes using sample prefractionation by solution
isoelectrofocusing prior to two-dimensional electrophoresis.
Anal Biochem 284, 266–278. Appendix C
Ordering Information
Catalog # Description
Protein Sample Preparation Kits and Reagents
Protein Extraction
163-2141
MicroRotofor ™ Cell Lysis Kit (Mammal), 15 preps,
includes 50 ml protein solubilization buffer (PSB),
ReadyPrep
™ mini grinders (2 packs of 10 each)
163-2142 MicroRotofor Cell Lysis Kit (Plant), 10 preps,
includes 50 ml protein solubilization buffer (PSB),
ReadyPrep 2-D cleanup kit (50 reaction size)
163-2143 MicroRotofor Cell Lysis Kit (Yeast), 15 preps,
includes 50 ml protein solubilization buffer (PSB),
15 ml yeast suspension buffer, 2 × 0.5 ml lyticase
(1.5 U/µl)
163-2144 MicroRotofor Cell Lysis Kit (Bacteria), 15 preps,
includes 50 ml protein solubilization buffer (PSB),
25 ml bacteria suspension buffer, 1 ml lysozyme
(1,500 U/µl)
163-2086 ReadyPrep ™ Protein Extraction Kit (Total
Protein), 20 preps, general purpose protein
preparation kit, includes strong detergent ASB-14
163-2146 ReadyPrep Mini Grinders, pkg of 20, 1.5 ml
grinding tube, contains grinding resin and matching
pestle, sufficient for twenty 100 mg extractions
163-2083 ReadyPrep 2-D Rehydration/Sample Buffer 1,
10 ml, protein solubilization reagent, includes 7 M
urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001%
bromophenol blue
163-2106 ReadyPrep 2-D Starter Kit Rehydration/Sample
Buffer, 10 ml, protein sample buffer, includes 8
M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte
®
ampholyte, 0.001% bromophenol blue
163-2145 Protein Solubilization Buffer (PSB), pkg of 1,
strongly chaotropic protein solubilization buffer,
contains NDSB 201, urea, thiourea, and CHAPS,
makes 50 ml solution
163-2091 ReadyPrep Proteomics Grade Water, 500 ml
161-0730 Urea, 250 g
161-0719 Tris, 1 kg
161-0460 CHAPS, 1g
161-0611 Dithiothreitol (DTT), 5 g
163-2101 Tributylphosphine (TBP), 0.6 ml, 200 mM
163-2109 Iodoacetamide, 30 g
161-0404 Bromophenol Blue, 10 g
Protein Sample Cleanup
163-2130
ReadyPrep 2-D Cleanup Kit, 50 preps
163-2140 ReadyPrep 2-D Cleanup Kit, 5 preps
732-6221 Micro Bio-Spin ™ 6 Columns, includes 25 columns
in Tris buffer, 50 collection tubes
732-6227 Bio-Spin ® 6 Columns, includes 25 columns in
Tris buffer, 50 collection tubes
732-6228 Bio-Spin 6 Columns, includes 100 columns in
Tris buffer, 200 collection tubes
163-2090 ReadyPrep Reduction-Alkylation Kit, 100 preps
Catalog # Description
Protein Fractionation
163-2100
ReadyPrep Sequential Extraction Kit, 5–15 preps
163-2085 ReadyPrep Protein Extraction Kit
(Soluble/Insoluble), 20 preps
163-2089 ReadyPrep Protein Extraction Kit
(Cytoplasmic/Nuclear), 50 preps
163-2088 ReadyPrep Protein Extraction Kit (Membrane I),
50 preps
163-2084 ReadyPrep Protein Extraction Kit (Membrane II),
10 preps
163-2087 ReadyPrep Protein Extraction Kit (Signal),
50 preps
732-6711 Aurum ™ CEX Mini Kit, 2 preps
732-6710 Aurum AEX Mini Kit, 2 preps
170-2800 MicroRotofor ™ Cell Kit, 100/120 V
170-2801 MicroRotofor Cell Kit, 220/240 V
170-2986 Rotofor ® Purification System, 100/120 V
170-2987 Rotofor Purification System, 220/240 V
170-2926 Model 491 Prep Cell, 100/120 V
170-2927 Model 491 Prep Cell, 220/240 V
170-2908 Mini Prep Cell without Reagent Starter Kit
Protein Sample Depletion
732-6701
Aurum Serum Protein Mini Kit, 10 preps
732-6712 Aurum ™ Affi-Gel ® Blue Mini Kit, 2 preps
163-3006 ProteoMiner ™ Protein Enrichment Small-
Capacity Kit, 10 preps for 10 mg total protein
163-3007 ProteoMiner Protein Enrichment Large-
Capacity Kit, 10 preps for 50 mg total protein
Protein Assay Kits and Instruments
500-0001 Bio-Rad Protein Assay Kit I, includes 450 ml dye
reagent concentrate, bovine
g-globulin standard;
sufficient for 440 standard assays or 2,200
microplate assays
500-0002 Bio-Rad Protein Assay Kit II, includes 450 ml
dye reagent concentrate, bovine serum albumin
standard; sufficient for 440 standard assays or
2,200 microplate assays
500-0111 DC ™ Protein Assay Kit I, includes 250 ml alkaline
copper tartrate solution, 2 L dilute Folin reagent,
5 ml surfactant solution, bovine
g-globulin standard;
sufficient for 450 standard assays
500-0112 DC Protein Assay Kit II, includes 250 ml alkaline
copper tartrate solution, 2 L dilute Folin reagent, 5 ml
surfactant solution, bovine serum albumin standard;
sufficient for 450 standard assays
500-0120 RC DC ™ Protein Assay Reagents Package,
includes RC reagents package and DC reagents
package, and sufficient for 450 standard assays
500-0121 RC DC Protein Assay Kit I, includes RC reagents
package, DC reagents package, bovine g-globulin
standard; sufficient for 450 standard assays
500-0122 RC DC Protein Assay Kit II, includes RC reagents
package, DC reagents package, bovine serum
albumin standard; sufficient for 450 standard assays
500-0201 Quick Start ™ Bradford Protein Assay Kit 1,
includes 1× dye reagent (1 L), bovine serum albumin
standard (5 × 2 mg/ml); sufficient for 200 standard
assays or 4,000 microplate assays
Related Bio-Rad Literature
Bio-Rad Laboratories, Inc. (2005) 2-D gel electrophoresis
troubleshooting. BioRadiations 116, 29–29.
Bulletin 1069 Colorimetric Protein Assays
Bulletin 2414 The Little Book of Standards
Bulletin 2587 High-Performance 2-D Gel Electrophoresis Using
Narrow pH-Range ReadyStrip IPG Strips
Bulletin 2895 Protein Blotting Guide
Bulletin 2998 Protein Standards Application Guide
Bulletin 3103 Removal of Abundant Myofilament Proteins from Rabbit
Myocardium Using the ReadyPrep Protein Extraction Kit (Membrane I)
Bulletin 3110 Tips to Prevent Streaking on 2-D Gels
Bulletin 3131 The Rotofor System As a Prefractionation Device Used
Prior to Electrophoresis
Bulletin 3133 Molecular Weight Determination by SDS-PAGE
Bulletin 3144 Using Precision Plus Protein Standards to Determine
Molecular Weight
Bulletin 3145 Strategies for Protein Sample Preparation
Bulletin 5241 Important Factors Influencing Protein Solubility for
2-D Electrophoresis
Bulletin 5344 Fractionation by Liquid-Phase Isoelectric Focusing in
the MicroRotofor Cell: Improved Detection of Low-Abundance Proteins
Bulletin 5398 Enriching Basic and Acidic Rodent Brain Proteins with
Ion Exchange Spin Columns for Two-Dimensional Gel Electrophoresis
Bulletin 5754 Comparison of SYPRO Ruby and Flamingo Fluorescent
Gel Stains with Respect to Compatibility with Mass Spectrometry
Bulletin 5782 In-Gel Protein Quantitation Using the Criterion Stain Free
Gel Imaging System
Bulletin 5841 Quantitation of Serum and Plasma Proteins after
Enrichment of Low-Abundance Proteins with the ProteoMiner Protein
Enrichment System
Bulletin 5911 Mini-PROTEAN TGX Precast Gel: A Versatile and
Robust Laemmli-Like Precast Gel for SDS-PAGE
Bulletin 5939 Overcoming the Coomassie Blues
Bulletin 6040 A Guide to Polyacrylamide Gel Electrophoresis
and Detection
Bulletin 6138 PROTEAN i12 IEF System: Independent Voltage
and Current Control Enables Optimization of First-Dimension
IEF Conditions
Bulletin 6139 Versatile Separation Capabilities of the
PROTEAN i12 IEF System
Bulletin 6140 Use of the PROTEAN i12 IEF System for In-Gel
Peptide Fractionation Prior to LC-MS and Comparison with
Off-Gel Fractionation
Bulletin 4006173 Instruction Manual, SYPRO Ruby Protein Stains
Bulletin 4307051 Instruction Manual, Bio-Safe Coomassie Stain
Bulletin 10003321 Instruction Manual, Flamingo Fluorescent Gel Stain
Bulletin 10017295 Instruction Manual, Oriole Fluorescent Gel Stain
Bulletin 10022069 Instruction Manual, PROTEAN i12 IEF System
Bulletin LIT-442 Instruction Manual, Silver-Stain Plus.