Bio-Rad GS-900™ Calibrated Densitometer - Manual (Page 48)

24 cm pH 5.5–6.7
24 cm pH 6.3–8.3
24 cm pH 7–10 R
24 cm pH 7–10 G
24 cm pH 3–10 R
24 cm pH 3–10 NL R
24 cm pH 4–7 R
24 cm pH 5–8 R
24 cm pH 3–10 G
24 cm pH 3–10 NL G
24 cm pH 4–7 G
24 cm pH 5–8 G
24 cm pH 3–6 R
24 cm pH 3.9–5.1
24 cm pH 4.7–5.9
24 cm pH 3–6 G
Step Voltage, V Ramp Time Units
1 10,000 Rapid 60,000 Vh
2 1,500 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 60,000 Vh
4 1,500 Hold
1 10,000 Rapid 44,000 Vh
2 1,500 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 44,000 Vh
4 1,500 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 70,000 Vh
4 1,500 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 70,000 Vh
4 1,500 Hold
1 10,000 Rapid 63,000 Vh
2 1,500 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 63,000 Vh
4 1,500 Hold
Table 9.5. Preprogrammed protocols for 24 cm ReadyStrip IPG strips.
R = rapid, G = gradual
Protocol Name Step Voltage, V Ramp Time Units
1 10,000 Rapid 43,000 Vh
2 1,000 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 43,000 Vh
4 1,000 Hold
1 10,000 Rapid 32,000 Vh
2 1,000 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 32,000 Vh
4 1,000 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 50,000 Vh
4 1,000 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 63,000 Vh
4 1,000 Hold
1 10,000 Rapid 46,000 Vh
2 1,000 Hold
1 250 Rapid 0:30 HH:MMr
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 46,000 Vh
4 1,000 Hold
17 cm pH 3–10 R
17 cm pH 3–10 NL R
17 cm pH 4–7 R
17 cm pH 5–8 R 18 cm pH 3–10 R
18 cm pH 3–10 NL R
18 cm pH 4–7 R
18 cm pH5–8 R
17 cm pH 3–10 G
17 cm pH 3–10 NL G
17 cm pH 4–7 G
17 cm pH 5–8 G 18 cm pH 3–10 G
18 cm pH 3–10 NL G
18 cm pH 4–7 G
18 cm pH 5–8 G
17 cm pH 3–6 R 18 cm pH 3–6 R
17 cm pH 3–6 G 18 cm pH 3–6 G
17 cm pH 3.9–5.1
17 cm pH 4.7–5.9 18 cm pH 3.9–5.1
18 cm pH 4.7–5.9
17 cm pH 5.5–6.7
17 cm pH 6.3–8.3 18 cm pH 5.5–6.7
18 cm pH 6.3–8.3
17 cm pH 7–10 R 18 cm pH 7–10 R
17 cm pH 7–10 G 18 cm pH 7–10 G
Table 9.4. Preprogrammed protocols for 17 and 18 cm ReadyStrip IPG strips.
R = rapid, G = gradual
Protocol Name
92 93
2-D Electrophoresis Guide Methods Chapter 9: First-Dimension IEF with IPG Strips
IEF Programming Recommendations
(contd.)