Bio-Rad GS-900™ Calibrated Densitometer - Manual (Page 27)

* All gel percentages listed in bold are available in IPG and/or prep-well comb format.
Criterion*
(for 11 cm IPG Strips)
Criterion Tris-Tricine Best choice for
16.5%
separation of 10–20%
low MW proteins
Criterion XT Bis-Tris Offer long shelf life, 10%
but require dedicated 12%
sample and running 4–12%
buffers
Gel Format
and Formulation Selection Criteria Composition Migration (%T)
Gel Format
and Formulation Selection Criteria Composition Migration (%T)
Criterion*
(for 11 cm IPG Strips)
Criterion Stain Free
Reagents are easy to 10%
Tris-HCl prepare, inexpensive 4–20%
and readily available 8–16%
Best choice when
switching between
precast and handcast
gels and when
comparing results
Stain-Free formulation
includes an additive
for rapid fluorescence
detection without
staining
Precast gels for Second-Dimension SDS-PAGE
(contd.)
4–12% 12% 10% 4–12% 12% 10%
Size, kD
(XT MOPS running buffer)
Size, kD
(XT MES running buffer)
26.6
17.0
6.5
3.5
1.4
14.0
26.6
17.0
6.5
3.5
1.4
14.0
10–20% 16.5%
250
150
100
50
25
75
37
20
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
10% 4–20% 8–16%
3–8% 7%
Size, kD
(XT Tricine running buffer)
Criterion XT Offer best resolution of 7%
Tris-Acetate high molecular weight 3–8%
proteins, but require
dedicated sample and
running buffers
50 51
2-D Electrophoresis Guide Theory and Product Selection Chapter 4: The Second Dimension: SDS-PAGE