Precast gels for second-dimension sds-page – Bio-Rad GS-900™ Calibrated Densitometer User Manual
Page 26
Gel Format
and Formulation
Selection Criteria
Composition
Migration (%T)
Gel Format
and Formulation
Selection Criteria
Composition
Migration (%T)
Mini-PROTEAN TGX Laemmli-like extended Any kD
shelf life gels
7.5%
10%
Best choice when
12%
long shelf life is
4–15%
needed and traditional 4–20%
Laemmli separation
patterns are desired
Mini-PROTEAN*
(for 7 cm IPG Strips)
Mini-PROTEAN TGX Laemmli-like extended Any kD
Stain-Free
shelf life gels
7.5%
10%
Best choice when
12%
long shelf life is
needed and traditional
Laemmli separation
patterns are desired
Stain-Free formulation
includes an additive
for rapid fluorescence
detection without
staining
Criterion Tris-HCl
Reagents are easy to 5%
prepare, inexpensive, 7.5%
and readily available
10%
12.5%
Best choice when
15%
switching between
18%
precast and handcast 4–15%
gels and need to
4–20%
compare results
8–16%
10–20%
10.5–14%
Mini-PROTEAN
Best choice for
16.5%
Tris-Tricine
separation of low
10–20%
MW proteins
Criterion*
(for 11 cm IPG Strips)
Criterion TGX
Laemmli-like extended Any kD
shelf life gels
7.5%
10%
Best choice when
12%
long shelf life is
18%
needed and traditional 4–15%
Laemmli separation
4–20%
patterns are desired
8–16%
10–20%
Criterion TGX
Laemmli-like extended Any kD
Stain-Free
shelf life gels
7.5%
10%
Best choice when
12%
long shelf life is
18%
needed and traditional 4–15%
Laemmli separation
4–20%
patterns are desired
8–16%
10–20%
Stain-Free formulation
includes an additive
for rapid fluorescence
detection without
staining
Precast Gels for Second-Dimension SDS-PAGE
(contd.)
* All gel percentages listed in bold are available in IPG and/or prep-well comb format.
250
150
100
75
50
37
25
20
15
10
250
100
150
75
50
37
25
20
15
250
150
100
75
50
37
25
20
250
150
100
50
25
75
37
20
15
10
250
150
100
75
50
37
20
25
15
10
250
150
100
75
50
37
7.5%
10%
12%
4-15%
4-20%
Any kD
250
150
100
50
25
75
37
20
250
150
100
50
25
75
37
20
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
4 –20%
4–15%
18%
7.5%
10%
12%
10 –20%
Any kD
8 –16%
250
150
100
50
25
75
37
20
250
150
100
50
25
75
37
20
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
10
15
4 –20%
4–15%
18%
7.5%
10%
12%
10 –20%
Any kD
8 –16%
250
150
100
75
50
37
25
20
15
10
250
100
150
75
50
37
25
20
15
250
150
100
75
50
37
25
20
250
150
100
75
50
37
7.5%
10%
12%
Any kD
25
20
15
10
5
2
25
20
15
10
5
2
10–20%
16.5%
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
10
250
150
100
50
25
75
37
20
15
250
150
100
50
25
75
37
250
150
100
50
75
37
250
150
100
75
18%
15%
12.5%
5%
7.5%
10%
4–20% 8–16% 10 –20% 10.5–14%
4–15%
48
49
2-D Electrophoresis Guide
Theory and Product Selection
Chapter 4: The Second Dimension: SDS-PAGE