1a f, Mm a – Eppendorf D30 BioPhotometer User Manual
Page 79
79
Evaluation procedure
Eppendorf BioPhotometer
®
D30
English (EN)
12.5.3
Calculating the factor for protein in "General Method Parameter"
This section only covers the calculation of the protein component in the
Proteins direct UV method group.
For this method group, the protein component is selected in the parameters (see Method parameters on
p. 32). The protein component is assigned a factor that will be entered in the
General Method Parameter/
Proteins function for each protein. Alternatively, A
0.1%
or the absorbance coefficient plus the molar mass
of the protein can be entered instead of the factor. In this case, the factor is calculated as follows:
F = factor for the protein; unit: g/L.
A
0.1%
= absorbance of the protein at a concentration of 0.1 % (1 g/L).
When entering the molar absorbance coefficient and the relative molar mass of the protein A
0.1%
can be
calculated on this basis:
ε
P
= molar absorbance coefficient of the protein; unit: cm
-1
M
-1
.
MM
P
= relative molar mass of the protein; unit: Da (entry in
General Method Parameter in kDa).
%
1
.
0
1
A
F
P
P
P
MM
A
H
%
1
.
0