Eppendorf D30 BioPhotometer - Manual (Page 33)

33
Methods
Eppendorf BioPhotometer ® D30
English (EN)
Standards Value input:
Number of standards.
Range: 1 to 12. Number of different standard concentrations for the evaluation
with standards.
For some methods the range for the number of standards is
restricted to a smaller range than 1 to 12.
Replicates Value input:
Number of replicates
per standard.
Range: 1 to 3. Number of repeated measurements for the various standard
concentrations.
Std. conc. Value input:
Concentration values
of the standards.
Limit: max. of 6 digits
including decimal
point. Based on the number of standards, this parameter is available
for all standards (e.g.,: std. conc. 1, std. conc. 2, ...).
Decimal places Value input:
Number of decimal
points for the result.
Range: 0 to 3. Number of decimal points for the calculated concentration
result.
Show scan Selection:
on | off Only for the Nucleic acids and Proteins direct UV method
groups: Display of a restricted scan
(absorbance-wavelength-graph with distances of 3 nm) in
addition to the result of the sample measurement.
A260/A280 Selection:
on | off Only for nucleic acids.
Display of the A260/A280 ratio in addition to the result of the
sample measurement.
A260/A230 Selection:
on | off Only for nucleic acids.
Display of the A260/A230 ratio in addition to the result of the
sample measurement.
Background Selection:
on | off Only for the Nucleic acids and Proteins direct UV method
groups:
Prior to the calculation of the results of a sample the
absorbance of a background wavelength, during which the
analyte to be measured should exhibit the absorbance value
zero, is subtracted from the absorbance of the measuring
wavelength. Frequent application: Partial correction of
turbidity for measurement of nucleic acids (background
wavelength in this case: 320 nm or 340 nm).
Wavelength Selection: 320 |
340 nm Wavelength at which the background is to be measured. The
analyte to be measured should have the absorbance value zero
in pure form here.
Parameter Entry Explanation