Eppendorf D30 BioPhotometer - Manual (Page 78)

Evaluation procedure
Eppendorf BioPhotometer
® D30
English (EN)
78
12.5.2.2 Calculation of the molar concentration
Application: calculation of the molar concentration of the nucleic acid from the mass concentration and
relative molar mass. The molar mass is either entered directly or calculated by the device from the entered
number of bases or base pairs per nucleic acid molecule.
C
Mol
= calculated molar concentration of the nucleic acid. Unit: pmol/mL.
C = nucleic acid concentration calculated from the measurement. Unit: μg/mL or ng/μL.
MM = relative molar mass. Unit: kDa
If the number of bases or base pairs per nucleic acid molecule are entered in More calculations instead of
the relative molar mass, the MM is calculated from the number of the bases or base pairs:
For dsDNA:
For ssDNA, RNA, Oligo:
MM = calculated relative molar mass; unit: kDa
bp = entered number of base pairs per molecule
b = entered number of bases per molecule
• For dsDNA the calculation of the molar concentration is based on the assumption of a
double-stranded nucleic acid. For the ssDNA, RNA and Oligo methods, a single-stranded
nucleic acid is assumed.
• For methods which have been reprogrammed via in the Routine main
group, Nucleic acids method group, always double-stranded nucleic acids are assumed
for calculating the molar concentration.
MM
C
C
Mol
3 10
u