Eppendorf D30 BioPhotometer - Manual (Page 23)

23
Operation
Eppendorf BioPhotometer ® D30
English (EN)
5.3 Summary of the measuring procedure
5.3.1 Preparing the measurement
1. Switch on the device and, if required, the printer.
The device performs a self test (taking approx. 1 minute) and displays the method selection.
2. Make ready the cuvettes for the measurements (see Inserting the cuvette on p. 22).
3. Prepare the measuring solutions for measuring the blank values, if required, also the standards and the
samples.
4. Open the cover of the cuvette shaft. The cover can remain open during the measurements.
5.3.2 Measuring procedure
5.3.2.1 Selecting a method
You should not use any measuring solution for standards and samples with a lower
absorbance than 0.02 to 0.03 A (e.g. dsDNA concentration between 1.0 and 1.5 μg/mL). The
detection limit of the device may be significantly lower, nevertheless, the impact of
disturbances from the measuring solutions (particles, bubbles, turbidity) on the reliability of
the result is very high for these low absorbance values.
 Use the cursor keys to select the desired
method and call up the method with the enter
key.
For an overview and a detailed description of the
methods, refer to the next chapter (see Methods on
p. 29).
Wizard: The wizard at the top of the display will
take you through the method procedure
step-by-step.
Help box: You will receive help texts in the lower
right of the display during each step of the
procedure.
Softkeys: The [< Back] and [Next >] softkeys allow
you to move between method steps in the wizard.