Eppendorf D30 BioPhotometer - Manual (Page 55)

55
Maintenance
Eppendorf BioPhotometer ® D30
English (EN)
8.3.1 Checking the photometer unit
Eppendorf offers a filter kit for checking the photometric accuracy and the wavelength systematic error.
The kit contains one blank filter A0 and 3 filters A1, A2 and A3 for checking the photometric accuracy as
well as 2 filters for checking the wavelength systematic error (260 nm, 280 nm). The filter absorbances are
measured in comparison with the blank filter A0. In addition to the information on the accuracy, you also
receive information on the precision: From the respectively 15 measurements per wavelength the variation
coefficient (cv-value) is determined next to the average value.
For measuring, first insert the blank filter (for the blank measurement) and then the test filter like cuvettes
into the cuvette shaft. The absorbance values measured for the test filters are compared with the allowable
value range. For the individual filters, the limit values for the permitted area are printed in a table in the lid
of the filter box.
Please connect the thermal printer if you would like to document the values.
Abb. 8-1: Inner lid of the filter box (model)
Fig. 8-1: Inner lid of the filter box (model)
BioPhotometer D30 reference filter set
SN: 6133 914.006 916.006 917.006 921.006 922.006 923.006
Filter Blank Sample Sample Sample Sample Sample
Type A 0 260 nm 280 nm A 1 A 2 A 3
260 nm 0.000 1.291-1.499 -- 0.197-0.221 1.078-1.101 1.857-1.888
280 nm 0.000 -- 1.041-1.287 0.193-0.217 1.024-1.047 1.717-1.749
320 nm 0.000 -- -- 0.173-0.196 0.923-0.947 1.478-1.509
405 nm 0.000 -- -- 0.161-0.184 0.903-0.926 1.429-1.461
562 nm 0.000 -- -- 0.172-0.195 0.941-0.968 1.496-1.530
595 nm 0.000 -- -- 0.172-0.195 0.943-0.969 1.503-1.538
≤ 3.0 % ≤ 2.0 % ≤ 1.5 %
550 - 600 nm ≤ 3.0 % ≤ 2.0 % ≤ 3.0 %
SRM 2031a: SN 667, valid through/gültig bis 12/2014
The valid certificates are part of the requalification documentation.
Die Gültigkeitszertifikate sind Teil der Qualifizierungsdokumentation.
Please protect against dust, heat, cold and liquid.
The limits are valid for max. 2 years. __________________________
e r u t a n g i S e t a D . n e z t ü h c s n e t i e k g i s s ü l F d n u e t l ä K , e z t i H , b u a t S r o v e t t i B
t f i r h c s r e t n U m u t a D . e r h a J 2 . x a m r ü f n e t l e g e t r e w z n e r G e i D
≤ 3.0 %
Wavelength and photometric accuracy/Wellenlängen- und photometrische Richtigkeit
traceable to/rückführbar auf
NIST SRM 2034, SN 04-A und SN 667
Zufällige Messabweichung des Photometers
Function : Device calibration/Photometer unit Order No./Best.Nr.: 6133 928.004
Set No./Satz Nr.: 006
Random error of wavelength
Zufällige Messabweichung der Wellenlänge
Limits measured against Blank A 0 at approx. 20°C
Grenzwerte gemessen gegen Blank A 0 bei ca. 20°C
Wavelength and photometric characterization of filters:
Random error of photometer
Limiting values (A)/Grenzwerte (E)
Limiting values CV (%)/Grenzwerte VK (%)
All characterization is performed on a Cary 100 Bio reference UV-Vis spectrophotometer, SN EL 99023107.
The instrument is requalified regularly by the manufacturer, and is confirmed and documented to
perform within manufacturer's specifications. The current instrument qualification is valid through April 2015.
260 - 405 nm ≤ 3.0 %
spektrometrische Sekundärstandards:
Die aktuelle Qualifizierung ist bis April 2015 gültig und verwendet folgende, NIST-rückführbare und zertifizierte,
Seriennummer EL99023107 durchgeführt.
Dieses Instrument wird regelmäßig vom Hersteller requalifiziert und die spezifikationsgemäße Funktion dokumentiert.
6133 928.020-00
For the current instrument qualification, the following, NIST traceable, certified secondary spectrometric
calibration standards were used:
Wellenlängen- und photometrische Bestimmung der Filter:
Alle Messungen werden auf einem Cary 100 Bio Referenz-UV-Vis-Spektrophotometer,