Eppendorf D30 BioPhotometer - Manual (Page 32)

Methods
Eppendorf BioPhotometer
® D30
English (EN)
32
6.3 Method parameters
This chapter illustrates the parameters for programming the methods. The order of the parameters in the
device display may slightly differ from the order in the table in order to display the parameters more clearly.
The table displays all parameters available for the various methods. Only a small portion of these
parameters are required for the corresponding method and will be shown in the display.
Parameter Entry Explanation
Cuvette Selection:
10 | 5 | 2 | 1 | 0.5 | 0.2 |
0.1 mm Optical path length of the cuvette. The device always
automatically converts absorbance values to the 10 mm path
length of a standard cuvette (see Absorbance values on p. 73).
Therefore, there is no need to change factors such as "50" for
the calculation of dsDNA concentrations when modifying the
Cuvette parameter.
Wavelength Selection:
230 | 260 | 280 | 320 |
340 | 405 | 490 | 562 |
595 | 600 nm Measurement wavelength: The concentration is calculated
based on the absorbance measured with this wavelength.
For some method groups (e.g.,
Nucleic acids and Proteins
direct UV), the wavelengths are preprogrammed.
Unit Selection:
mg/mL | μg/mL | ng/
mL | pg/mL | μg/μL |
mg/dL | μmol/mL |
nmol/mL | pmol/mL |
pmol/μL | U | U/mL |
U/L | % | Abs | A/min
In addition, further
units are freely
programmable in the
General Method
Parameters/Units
function. Max. 7 digits. Unit for the concentration result.
In the preprogrammed methods of the Routine group, the
selection is restricted to units that are useful for these methods.
Calculation Selection:
factor | standard Evaluation procedure for the calculation of the sample
concentration from the measured absorbance.
Factor Value input:
Factor.
Limit: max. of 6 digits
including decimal
point. Factor for converting absorbance values into the concentration.
For the
Factor method group, you also can enter negative
factors.
Protein Selection:
List of protein types
which are stored in the
General Method
Parameters/Proteins
function. Only for the Proteins direct UV method group.
When selecting the protein, the corresponding Factor
parameter programmed in the General Method Parameters/
Proteins function also will be imported from that function.