Eppendorf D30 BioPhotometer - Manual (Page 22)

Operation
Eppendorf BioPhotometer
® D30
English (EN)
22
5.2 Inserting the cuvette
Standard rectangular glass or plastic cuvettes can be inserted in the cuvette shaft:
• External dimensions: 12.5 mm × 12.5 mm
• Height of light path: 8.5 mm higher than cuvette base
• Total height: min. 36 mm
The cuvettes must be optically transparent for the respective measuring wavelength. For measurements in
the UV range, Eppendorf offers the plastic cuvette UVette which is transparent for wavelengths of 220 nm
and higher and therefore also is suitable for measuring nucleic acids.
Prerequisites
• The cuvette is free from contamination by dust or fingerprints and free from scratches.
• The cuvette shaft is free from particles, dust and liquid.
• The measuring volume in the cuvette is sufficient. Ensure that the minimum measuring volume has
been reached.
• The measuring solution is free from particles and bubbles.
• The cuvette temperature and temperature control of the cuvette are above the temperature of the dew
point that applies for the ambient conditions (humidity and temperature).
1. Position the cuvette so that the optical window of the cuvette is pointing towards the direction of the
light path.
2. When inserting the cuvette, press it completely to the bottom against the slight resistance.
The direction of the light path is marked with an arrow on the housing.
Cuvettes
* or similar microliter cuvette
See manufacturer
information
See manufacturer
information
Macro Semi-micro Ultra-micro UVette ® Hellma ® TrayCell *
50 μL 70 μL 400 μL 1000 μL
Eppendorf
μCuvette G1.0
Basic area 12.5 mm × 12.5 mm
Min. overall height 36 mm
Min. filling level 10 mm
Light path 8.5 mm
Max. height of base 7 mm
0 mm
Min. volume Photometry