2 technical data – Eppendorf BioPhotometer User Manual

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2 Technical data

Photometer

Optical system:

Absorption single-beam photometer with reference beam
and several ﬁxed wavelengths

Irradiation source:

Xenon ﬂash lamp

Spectral dispersion:

Holographic concave grating

Measuring wavelengths:

Xe 230, 260, 280, 320, 562, 595 nm

Wavelength selection:

Method-dependent, program-controlled

Spectral bandwidth:

5 nm at 230 to 320 nm
7 nm at 562 to 595 nm

Wavelength systematic error:

1 nm at 230 to 280 nm

2 nm at 320 to 595 nm

Wavelength random error:

≤

0.1 nm

Photometric measuring range:

Quartz glass cuvette: 0.000 to 3.000 A

UVette

(Eppendorf): 2.5 A at 230 nm

2.6 A at 260 nm
2.8 A at 280 nm
2.9 A at 320 nm

Photometric random error:

≤

0.002 A at 0 A

≤

0.005 A at 1 A

Photometric systematic error:

1 % at 1 A

Accuracy of reading:

0.001 A

Stray-light proportion:

< 0.05 %

Radiation detector:

Silicium photo diodes

Measuring procedures

Measuring procedure:

End-point against blank

Method-dependent
calculation:

Absorbance
Concentration via factor
Concentration via Warburg formula
Concentration via calibration with 1 to 10 standards
One-point calibration (1 standard)
Linear regression (2 to 10 standards)
Non-linear regression
(3rd degree polynomer; 4 or 5 to 10 standards;
see Section 12, "Calculation")
1 x, 2 x or 3 x determination

For nucleic acids:
Ratio 260/280
Ratio 260/230
Molar concentration
Total yield

Memory

Method memory:

12 preprogrammed, modiﬁable method programs

Calibration memory:

For all calibration procedures

Results memory:

For 100 results with absorbance and ratio values,
sample number, sample dilution, date and time (calendar up to 2090)

2 Technical data

02_TecDat_e.fm Seite 51 Montag, 20. Februar 2006 16:06 Uhr