5 operation – Eppendorf BioPhotometer User Manual
Page 18
60
5 Operation
Measure sample
Results display
As an indication of the purity of the nucleic acid sample which
has been measured, the absorbance at 230, 280 and 320 nm
as well as the ratios A
260
/A
280
and A
260
/A
230
are displayed in
addition to the concentration result and the absorbance at a
wavelength of 260 nm. With pure samples, the absorbance at
320 nm should be approximately zero.
Turbid measuring solutions show increased absorbances for
all wavelengths. These can adulterate the results. In such
cases the influence on the result can be partially corrected by
switching on the "Corr. with A
320
" parameter. (Chapter 6.3
"Explanation of parameters")
Measure
next sample
To measure the next sample, press the
key again.
Sample dilution
The sample dilution in the measuring cuvette can be entered
using the
key before the measurement starts and is
included automatically in the result calculation (see
"Measuring diluted samples").
Conversion key
The most-recently measured concentration result can be
converted into molar concentrations and/or into nucleic acid
quantities (unit of mass or unit of mol):
Entering "TOTAL SAMPLE"
The value entered is converted using the concentration
measured. The result shown is the quantity of nucleic acid
present in the sample.
Entering "BASE PAIRS" or "MOL.MASS"
It is sufficient to make an entry in only one of the two lines.
The molar concentration is calculated using the value
entered and the concentration measured.
Input fields can be skipped using the
key.
Sample
d s D N A
S A M P L E 0 0 1
0 . 6 9 4 A
2 3 0
1 . 4 0 8 A
2 6 0
1 . 9 7
2 6 0
/
2 8 0
0 . 7 1 5 A
2 8 0
2 . 0 3
2 6 0
/
2 3 0
0 . 0 0 2 A
3 2 0
7
0
.
0
µg/mL
Sample
Dilution
Conversion
C A L C. A M O U N T:
TOTA L S A M P L E –
–
–
–
µL
C A L C. M O L A R I T Y:
B A S E PA I R S
–
–
–
–
M O L . M A S S
–
–
–
– k D a
Enter
05_Bedienung_e.fm Seite 60 Dienstag, 21. Februar 2006 10:09 Uhr