Eppendorf BioPhotometer - Manual (Page 39)

81
10 Short instructions
Preparation
The BioPhotometer is ready to measure immediately after being switched on.
Methods
dsDNA ssDNA RNA Oligo
– Direct measurement of the nucleic acids at 260 nm.
– Ratios A
260 /A 280 and A 260 /A 230 .
– Optional correction of absorbance values via A 320.
– Measurement using quartz-glass cuvette or UVette
®
from Eppendorf.
OD 600
– Direct measurement of the density of bacteria suspensions
at 600 nm (turbidity measurement).
– Measurement using glass cuvette or plastic cuvette.
Protein
– Direct measurement of protein at 280 nm.
– Direct measurement of the absorbance, or calculation via factor,
standard or Warburg formula.
– Optional correction of absorbance values via A
320.
– Measurement using quartz-glass cuvette or UVette
®
from Eppendorf.
Bradford Lowry BCA
Bradford micro Lowry micro BCA micro
– Measurement of protein using Bradford-, Lowry- or BCA reagent.
– Direct measurement of the absorbance,
or calculation via factor or calibration
(single-point calibration, linear regression or non-linear regression).
– Number and nominal values of the calibrators are programmable.
– The protein methods are also available on a micro-scale
(Press the Method key twice).
– Measurement using glass cuvette or plastic cuvette.
8
ssDNA
7
dsDNA
9
RNA
6
Oligo
5
OD 600
4
Protein
1
Bradford
2
Lowry
3
BCA
7
dsDNA
8
ssDNA
9
RNA
6
Oligo
5
OD 600
4
Protein
1
Bradford
2
Lowry
3
BCA
Cuvettes
Min. overall height
Min. filling level
Min. volume
36 mm
Light path
Max. height of base
10 mm
8.5 mm
7 mm
0 mm
70 µL 400 µL 1000 µL 300 µL
Semi-micro Macro Suction
Basic area
12.5 mm x 12.5 mm
Ultra-micro UVette
®
50 µL
10_Kurz_e.fm Seite 81 Montag, 20. Februar 2006 11:33 Uhr