10 short instructions, Measuring procedure for dsdna – Eppendorf BioPhotometer User Manual
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10 Short instructions
Measuring procedure for dsDNA
d s D N A
P RO G R A M M E D FAC TO R
1 A 2 6 0 = 5 0 . 0
µg/mL
Sample
d s D N A
B L A N K
Blank
d s D N A
S A M P L E 0 0 1
2 0 + 2 0 0
µL
0
.
0
0
0
A
C A L C : A M O U N T:
TOTA L S A M P L E
1 4 0
µL
C A L C : M O L A R I T Y:
B A S E PA I R S
3 0 0
M O L . M A S S
1 9 8 k D a
d s D N A
S A M P L E 0 0 1
2 0 + 2 0 0
µL
0 . 6 9 4 A
2 3 0
1 . 4 0 8 A
2 6 0
1 . 9 7
2 6 0
/
2 8 0
0 . 7 1 5 A
2 8 0
2 . 0 3
2 6
0 /
2 3 0
0 . 0 0 2 A
3 2 0
5
6
3
.
2
0
µg/mL
d s D N A
S A M P L E 0 0 1
2 0 + 2 0 0
µL
7 9
µg
2 8 4 3 p m o l / m L
3 9 8 p m o l
5
6
3
.
2
0
µg/mL
d s D N A
S A M P L E 0 0 2
2 0 + 2 0 0
µL
0 . 6 8 9 A
2 3 0
0 . 7 8 8 A
2 6 0
1 . 8 5
2 6 0
/
2 8 0
0 . 6 2 3 A
2 8 0
2 . 1 9
2 6
0 /
2 3 0
0 . 0 0 3 A
3 2 0
2
4
9
.
7
0
µg/mL
Select dsDNA method
Measure blank
Enter
Enter
2
Lowry
Dilution
0
Sample No.
0
Sample No.
0
Sample No.
When the sample is diluted:
Example: 20 + 200
µL
2
Lowry
Measure sample
If the result of the sample
is to be converted:
Enter
Enter
Enter
Conversion
0
Sample No.
0
Sample No.
0
Sample No.
1
Bradford
4
Protein
3
BCA
Measure next sample
Sample
Sample
7
dsDNA
Blank
Blank
Sample
or
or
Sample
Blank
or
10_Kurz_e.fm Seite 83 Montag, 20. Februar 2006 11:33 Uhr