Eppendorf BioPhotometer - Manual (Page 42)

84
10 Short instructions
Bradford measuring procedure
B R A D F O R D
C A L I B R AT I O N R A N G E
1 0 0 – 2 0 0 0
µg/mL
Standard
B R A D F O R D B L A N K
Blank
0 . 0 0 0
A
B R A D F O R D S A M P L E 0 0 1
2 0 + 2 0 0 µL
0 . 3 5 2 A 5 9 5
3 6 8
µg/mL
B R A D F O R D S A M P L E 0 0 2
2 0 + 2 0 0 µL
0 . 5 2 5 A 5 9 5
5 5 2 µg/mL
Select Bradford method
Measure blank
Measure sample
Measure next sample Sample
Sample
Blank
1
Bradford
If "Bradford micro"
is to be selected:
B R A D. m i c r o
C A L I B R AT I O N R A N G E
1 . 0 – 2 0 . 0
µg/mL
Standard Blank
1
Bradford
B R A D F O R D S T D 6
2 0 0 0 µg/mL
C V: 2 . 8 %
C A L I B R AT I O N S TO R E D
1 . 3 2 5
A
B R A D F O R D S T D 1
1 0 0 µg/mL
N E X T: S T D 2
2 0 0 µg/mL
0 . 0 4 8
A
Measure last standard
Measure first standard
Enter
Enter
2
Lowry Dilution
0
Sample No.
0
Sample No.
0
Sample No.
When the sample is diluted:
Example: 20 + 200
µL
2
Lowry
Standard
Standard
B R A D F O R D S A M P L E 0 0 1
2 0 + 2 0 0 µL
Blank Standard
Sample
or
or
or
Blank Standard
Sample
or
10_Kurz_e.fm Seite 84 Montag, 20. Februar 2006 11:33 Uhr