Eppendorf BioPhotometer - Manual (Page 24)

66
5 Operation
5.5 Measuring OD 600
Select method
Measuring procedure
Blank measurements remain stored until the date changes
(for further details, see "Measuring nucleic acids").
Measure blank
Measure sample
Measure
next sample To measure the next sample, press the key again.
Sample dilution Sample dilution in the measuring cuvette can be entered
using the
key before the measurement begins and
is then included automatically in the result calculation
(see "Measuring diluted samples").
The OD 600 measurement is a stray-light measurement;
the result is therefore heavily dependent on the geometry
of the light path, which may vary between photometers
from different manufacturers.
5
OD 600
O D 6 0 0
Blank Sample or
Blank
O D 6 0 0 B L A N K
0 . 0 0 0
A
Blank Sample or
Sample
O D 6 0 0 S A M P L E 0 0 1
0 . 4 3 0
A
Sample
Dilution
05_Bedienung_e.fm Seite 66 Dienstag, 21. Februar 2006 10:09 Uhr