Eppendorf BioPhotometer - Manual (Page 30)

72
6 Programming
6.3 Explanation of parameters
Parameters are defined as selection parameters or as parameters for entering numbers. In the case of
selection parameters, the programmable alternatives are method-dependent (see overview in previous
section).
Parameter Entries Explanation
Calculation Selection Selection of calculation procedures: Absorbance, Factor,
Standard and Warburg formula.
In the case of calculation using the Warburg formula, the
measured value for A
260 is marked in the results display
and on the results printout with a " ".
Factor Entry of numbers
(five-figure) (Only when the calculation process "Factor" has been
selected)
Entering a factor;
the number of decimal places determines the number of
decimal places in the result.
Corr. with A 320 Selection (Only for nucleic acid methods and for the direct photometric
determination of protein)
Selection from "Corr. with A
320 off" and "Corr. with A 320 on";
"Corr. on" means: the absorbance measured at 320 nm is
subtracted from the absorbance results at 260, 280 and
230 nm. Example of application: Correction of turbidity in the
sample.
When the correction function is switched on, the measuring
value for A
320 is marked with a " " in the results display and
on the results printout.
Unit Selection The selection from preprogrammed concentration units is
method-dependent.
M. unit
(molar unit) Selection Selection is method-dependent
(for nucleic acid measurements only);
is required for the conversion of the concentration into molar
concentrations (
key).
Cuvette Selection Selection from 10 mm, 5 mm, 2 mm and 1 mm optical path
length; the result is converted for an optical path length of
10 mm (see Section 12 "Calculation").
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Conversion
06_Programm_e.fm Seite 72 Montag, 20. Februar 2006 10:54 Uhr