Eppendorf BioPhotometer - Manual (Page 48)

90
13 Testing the photometer
To enable the photometric accuracy and the wavelength accuracy to be tested, a filter set (secondary UV-VIS
filter) is available from Eppendorf. This set contains three filters ("Sample A
1 ", "Sample A 2 " and "Sample A 3 ")
for testing the photometric accuracy and two filters ("Sample 260 nm" and "Sample 280 nm") for testing the
wavelength accuracy. The absorbance of the filters is measured against a blank filter ("Blank A
0 ").
To carry out these measurements, blank filters and "sample filters" (test filters) are inserted into the cuvette
holder in the same manner as cuvettes. When doing so, please ensure that the label with the filter description
is facing the user. The absorbance values measured for the test filters are compared to those within the range
of permitted values. The limits for the permitted range are contained in a table found on the inside of the lid of
the filter box (see Figure: "X.XXX – X.XXX A").
Fig.: Inside of the lid of the filter box
BioPhotometer
Function: PHOTOMETERTEST
Secondary / Sekundär - UV - VIS - Filter Order No. / Best.Nr.: 6131 928.007
Limits
Grenzwerte measured against Blank A 0 at approx. 20 °C
gemessen gegen Blank A 0 bei ca. 20 °C
6131 914.XXX 916.XXX 917.XXX 921.XXX 922.XXX 923.XXX
Filter
Type
Blank
A 0
Sample
260 nm
Sample
280 nm
Sample
A 1
Sample
A 2
Sample
A 3
Reference Systematic error / Systematische Messabweichung
empty
leer
of wavelength / der Wellenlänge of photometer / des Photometers
Traceable to / rückführbar auf
Nist: SRM 2034, SN: 99 Nist: SRM 2031a, SN: 577
(A) / (E) Limiting values (A) / Grenzwerte (E)
230 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
260 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
280 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
320 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
562 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
595 nm 0.000 X.XXX - X.XXX X.XXX - X.XXX X.XXX - X.XXX
230 –
595 nm
Random error / Zufällige Messabweichung
of wavelength / der Wellenlänge of photometer / des Photometers
Limiting values CV (%) / Grenzwerte VK (%)
0.000 ≤ 3.0 % ≤ 3.0 % ≤ 1.0 % ≤ 1.5 %
Please protect against dust, heat and liquid
The limits are valid for max. 2 years.
Bitte vor Staub, Hitze und Flüssigkeiten schützen
Die Grenzwerte gelten für max. 2 Jahre.
Date Signature
Datum Unterschrift
eppendorf
13 Testing the photometer
13_Ueberpruef_e.fm Seite 90 Montag, 20. Februar 2006 11:35 Uhr