5 operation – Eppendorf BioPhotometer User Manual

Page 20

5 Operation

Measuring procedure

The following example shows the measuring procedure
for the "Absorbance" calculation mode. For details of the
measuring procedure via standard (one-point calibration),
please refer to "Measuring proteins with reagent".

Blank measurements remain stored until the date changes
(For further details, please refer to "Measuring nucleic
acids").

Measure blank

Measure sample

Results display

In addition to the concentration result and the absorbance at
the measuring wavelength of 280 nm, A

260

and A

320

appear

in the display as an indication of the purity of the sample. The
absorbance at 320 nm should be approximately zero.

Turbid measuring solutions show increased absorbances for
all wavelengths. These can adulterate the results. In such
cases the inﬂuence on the result can be partially corrected by
switching on the "Corr. with A

320

" parameter. (Chapter 6.3

"Explanation of parameters")

Measure
next sample

To measure the next sample, press the

key again.

Sample dilution

Sample dilution in the measuring cuvette can be entered
using the

key before the measurement begins and

is then included automatically in the following calculation of
sample concentrations (see "Measuring diluted samples").

Blank

P ROT E I N

B L A N K

Blank

Sample

P ROT E I N

S A M P L E 0 0 1

0 . 2 4 5 A

2 6 0

0 . 4 3 0 A

2 8 0

0 . 0 0 2 A

3 2 0

Sample

Dilution

05_Bedienung_e.fm Seite 62 Dienstag, 21. Februar 2006 10:09 Uhr