Eppendorf BioPhotometer - Manual (Page 8)

50
1 Overview
Methods There are twelve preprogrammed factory-set methods which can be called up at the
push of a button:
Nucleic acids
dsDNA Double-stranded DNA
ssDNA Single-stranded DNA
RNA RNA
Oligo Oligonucleotides
Proteins
Protein Direct photometric measurement
Bradford Bradford method
Bradford micro Bradford method, low concentration range
Lowry Lowry method
Lowry micro Lowry method, low concentration range
BCA BCA method
BCA micro BCA method, low concentration range
Bacteria density
OD 600 Turbidity measurement
Method Each method has an accompanying, factory-set program that contains different
parameters, such as units of concentration and type of calculation. The method
programs can be changed at any time using the
key. Before using a method for
the first time, call up the corresponding method program and – if necessary – adapt it to
suit your requirements. For methods which are to be calculated using calibration by
standard measurements, the number and nominal concentrations of the standards
must be adapted.
Measurement For measurement purposes, the desired method should be called up using the
appropriate measuring key. The Bradford, Lowry and BCA methods have the same
special feature: For each of these methods, two different calculation ranges may be
programmed. It is possible to toggle between the two method programs (e.g. "BCA"
and "BCA micro") by pressing the method key repeatedly.
Pressing one of the three oval measuring keys starts the measurement. The device is
ready to measure immediately after being switched on. An indication as to which of the
three measuring keys should be used for a measurement can be found in the lower part
of the device display (Details on the measuring process can be found in Section 5,
"Operation").
Calculation It is possible to calculate the result automatically using method-specific programmed
calculation modes (factor, calibration, Warburg formula or direct absorbance output). In
addition to the calculated results, the absorbances and (for nucleic acids) the common
absorbance ratios appear in the display.
Sample dilutions can also be included in the calculation process ( key). The
calculated mass concentrations for nucleic acids can be converted into molar
concentrations by pressing the
key. This key can also be used to calculate the
total sample quantity ("yield") in the sample vessel.
Results printout The results appear in the device display and can be printed out (if the printer is
connected). A data transfer program is available from Eppendorf for evaluating your
results on a computer using a calculation program (see Sec. 11, "Ordering
information").
Sample results and calibration results are stored; this data can be called up by pressing
the
key.
Parameter
Dilution
Conversion
•
Function
01_Ueberblick_e.fm Seite 50 Montag, 20. Februar 2006 10:47 Uhr