Eppendorf BioPhotometer - Manual (Page 22)

64
5 Operation
Measuring procedure
Blank measurements remain stored until the date changes
(For further details, see "Measuring nucleic acids").
Standard measurements remain stored until they are overwritten
with new standard measurements. For the calculation of sample
measurements, the most-recently stored calibration is used.
In the following example, multiple-point calibration with 5 standards
in double determination and calibration calculation via non-linear
regression was programmed as a calculation procedure for the
Bradford method:
Measure blank
Measure
standards Standard 1 /
first measurement
The first two lines of the display contain the standard that has just
been measured. The last two lines of the display contain the next
standard which is to be measured, with the nominal concentration.
Standard 1 /
second measurement
Blank
B R A D F O R D B L A N K
0 . 0 0 0
A
Blank Standard or
Sample
Standard
B R A D F O R D S T D 1 – 1
X X X X µg/mL
N E X T: S T D 1 – 2
X X X X µg/mL
X . X X X
A
Standard
B R A D F O R D S T D 1 – 2
X X X X µg/mL
N E X T: S T D 2 – 1
X X X X µg/mL
X . X X X
A
05_Bedienung_e.fm Seite 64 Dienstag, 21. Februar 2006 10:09 Uhr