Eppendorf BioPhotometer - Manual (Page 35)

77
8 Error messages, result flagging and help texts
Result flagging
Error texts in the results display
Flagging Explanation
1.586 A 260 Flagging of A 260 in the display or on the printout
(for method "Protein direct" only):
The method was calculated using the Warburg formula.
0.015 A 320 Flagging of A 320 in the display or on the printout
(for method "Protein direct" and for nucleic acid methods only):
The absorbances at 260, 280 and 230 nm are corrected with the absorbance
at 320 nm (see Section 6, "Programming").
Error text Explanation / Cause Solution
+++++ The absorbance measured is greater
than 3.0 A. – Dilute the sample.
– Check the cuvette
(height of light path must
be 8.5 mm).
– Clean the cuvette shaft
(see Section 9).
– Insert the cuvette correctly (the
measuring window must be facing
the light path).
– Use a cuvette made of material
that transmits light at the
measuring wavelengths used
(e.g. quartz glass or UVette
®
from
Eppendorf for nucleic acid
measurement).
! ! ! ! ! The calculated result cannot be displayed
(value too high). Check the parameter
(Is the factor too high?).
– – – – – (Instead of a value for the ratio:)
Ratio cannot be calculated because one of
the absorbance values used for calculating
the ratio is 0 A or > 3.0 A. Repeat the measurement
(dilute sample if necessary).
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08_Fehler_e.fm Seite 77 Montag, 20. Februar 2006 11:24 Uhr