Eppendorf BioPhotometer - Manual (Page 29)

71
6 Programming
6.2 Overview of parameters
(For "Factor" calculation only:)
(For "Standard" calculation only:)
Point 1: No selection possible; "Factor" calculation is preprogrammed .
Point 2: No selection possible; the unit "Absorbance" is preprogrammed.
Point 3: No selection possible; the number of standards "1" is preprogrammed .
Point 4: Selection possible only if at least "4" (or, for the single determination of the standard,
at least "5") has been entered for the "Std. number" parameter
.
dsDNA
ssDNA
RNA Oligo Protein Bradford
Brad.micro
Lowry
Low.micro
BCA
BCA micro OD 600
Calculation (Point 1) (Point 1) Absorbance
Standard
Factor
Warburg formula Absorbance
Standard
Factor (Point 1)
Correction
with A
320
Off
On Off
On Off
On
Unit µ g/mL
ng/ µ L
µ g/ µ L
µ g/mL
ng/ µ L
µ g/ µ L
mg/mL
µ g/mL
mg/mL
µ g/mL
µ g
(Point 2)
Molar unit pmol/ µ L
µ mol/L
pmol/mL
pmol/ µ L
µ mol/L
Cuvette 10 mm
5 mm
2 mm
1 mm 10 mm
5 mm
2 mm
1 mm 10 mm
5 mm
2 mm
1 mm 10 mm
5 mm
2 mm
1 mm 10 mm
5 mm
2 mm
1 mm
Factor Entry of
numbers Entry of
numbers Entry of
numbers Entry of
numbers Entry of
numbers
No. of
standards (Point 3) Entry of
numbers
Std.
measurement 1x
2x
3x 1x
2x
3x
Regression
(Point 4)
Linear
Non-linear
Standard Entry of
numbers Entry of
numbers
06_Programm_e.fm Seite 71 Montag, 20. Februar 2006 10:54 Uhr