Eppendorf BioPhotometer - Manual (Page 32)

74
6 Programming
6.4 F
actor
y-set pr
ogrammed v
alues
Notes:
1)
With"F
actor" calculation:
Input required from user
2)
With "Standard" calculation
3)
With "Standard" or "F
actor" calculation
4)
With "Standard" calculation:
Input required from user
dsDNA
ssDNA
RNA
Oligo
Pr
otein
Bradf
or
d
Bradf
or
d
micr
o
Lo
wr
y
L
o
w
ry
micr
o
BCA
BCA micr
o
OD 600
Calculation
Absorbance
Standard
Standard
Standard
Standard
Standard
Standard
F
actor
50.0
37.0
40.0
30.0
- - - - -
1)
- - - - -
1)
- - - - -
1)
- - - - -
1)
- - - - -
1)
- - - - -
1)
- - - - -
1)
1.000
Corr
. with A
320
off
off
off
off
off
Std.
n
umber
6
6
6
6
8
5
Std.
measur
mnt.
1x
2)
1x
1x
1x
1x
1x
1x
Reg
ression
non-linear
non-linear
non-linear
non-linear
non-linear
non-linear
Unit
µ
g/mL
µ
g/mL
µ
g/mL
µ
g/
µ
L
µ
g/mL
3)
µ
g/mL
µ
g/mL
µ
g/mL
µ g/mL
µ
g/mL
µ
g/mL
Molar unit
pmol/mL
p
mol/mL
p
mol/mL
pmol/
µ L
Standard 1
- - - - -
4)
100
1.00
100
1.00
25
0.50
Standard 2
250
2.5
250
2.5
125
2
Standard 3
500
5
500
5
250
5
Standard 4
750
10
750
10
500
10
Standard 5
1000
15
1000
15
750
20
Standard 6
1500
25
1500
25
1000
Standard 7
1500
Standard 8
2000
Cuv
ette
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
10 mm
06_Programm_e.fm Seite 74 Montag, 20. Februar 2006 10:54 Uhr