Eppendorf BioPhotometer - Manual (Page 49)

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13 Testing the photometer
Test procedure
– Carry out the test at approximately 20 ° C.
– Remove the filter from the filter box for a brief period only. Make sure that the surface of the filter is not
contaminated or damaged.
– Protect the filter from dust, heat, liquid and aggressive vapors.
– When inserting the filter, ensure that the label with the filter description is facing the user.
– Select the function "Photometer test".
This function is contained in devices with a software version of V 1.20 onwards. Contact Eppendorf before
using the test filter with an older software version.
– Select the test filter.
– "A 1 ", "A 2 " or "A 3 " for the measurement of the photometric accuracy at 230, 260, 280, 320, 562
and 595 nm.
– "A 260 " or "A 280 " for the measurement of the wavelength accuracy at 260 or 280 nm.
– Follow the instructions in the photometer display for the measurement of "Blank" and "Sample".
The device carries out 10 measuring cycles and then prints out the mean values for the absorbances at
the respective wavelengths.
– Compare the absorbance values with the permitted value range.
– In addition to information on accuracy values, the printout contains details of precision as well. The mean
value and the CV are calculated from each of the ten measurements.
If the absorbances measured are not within the permitted value range, please contact the Service Depart-
ment at Eppendorf. The filters should be recalibrated by Eppendorf after two years.
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