Multichannel Systems Roboocyte2 Manual User Manual

Page 96

Roboocyte2 Manual

In order to have a closer look at the recordings in the “Data List”, click on the triangle icon

"Expand / Collapse All"

left to the well entry. In this example, seven recordings have been

performed to generate a dose-response curve for the P2X2 receptor that is an ATP-gated,
on-selective cation channel. To expand all well entries simultaneously, click on the icon

"Select / Deselect All"

to select and display all recordings in the plate. Another click

on the respective icon works in the opposite direction.

Data List: "Results" tabbed Page

The "Results" tab includes the following information, from left to right:

 Well: The well number for the recording (B12).

 ID: The recording ID (from 30 to 36).

 Series: The series number; if no series has been assigned in the recording script, series value

is zero.

 ROI: The ROI (Region of Interest) number.

 Minimum: The minimum current within the ROI.

 Pos Min: The position (time) within the ROI where the minimum was detected (in ms, s, or min).

 Maximum: The maximum current within the ROI.

 Pos Max: The position (time) within the ROI where the maximum was detected (in ms, s, or min).

 Average: The average current within the ROI.

 Area: The calculated area (charge) within the ROI in nA*s. The area is calculated from absolute

current; please note that this calculation gives meaningful values only after subtracting the
baseline.

 Left: Position of the left ROI cursor.

 Right: Position of the right ROI cursor.