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3 manual mode measurement – Multichannel Systems Roboocyte2 Manual User Manual

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Manual Mode

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5.3 Manual

Mode

Measurement

The "Measurement" tab page of the "Manual Mode"

The "Measurement" tab page of the "Manual Mode" is used to perform TEVC experiments
"manually". Manually means that - although everything is still controlled by software - all steps
like movements of carrier and z-axis, impalement, voltage clamp, solution exchanges can be
realized with single commands under full control by the experimenter.

For different purposes it is useful to make experiments manually:

 To test the quality of oocytes before starting automated, script-controlled experiments.

 To test the expression before going automatic.

 To test new IV protocols or solution exchange sequences before implementing them into a script.

 To find an optimal impalement procedure for a given batch of oocytes.