Multichannel Systems Roboocyte2 Manual - Manual (Page 82)

Roboocyte2 Manual
78
4. Start the recording in current clamp mode with Clamp Current 0 nA. This will display the voltage
of Current and Voltage electrode in green and blue, respectively. Uncompensated voltage offsets
should be around -70 mV with electrodes filled with 3 M KCl. Wait until the offsets are constant
and:
Press the DC Offset button to compensate the electrode offsets.
The electrode voltages should be close to 0 mV after offset compensation.
Electrode voltages should be close to 0 mV and constant over time after performing offset
compensation.
Press the Oocyte button to move the measuring head further down into the oocyte.
The "Oocyte position" can be defined in the "Options" menu. Default value is 800 μm,
which means 800 μm above well plate bottom.