Multichannel Systems Roboocyte2 Manual - Manual (Page 70)

Roboocyte2 Manual
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For often needed solutions like Ringer's solution or reference compounds, it is recommended you
use the transfer ports of the liquid handler. To the transfer ports large bottles can be connected.
Please make sure to use the provided needle with the beveled tip if you plan to use the transfer
ports of the Gilson GX-271. Otherwise, the transfer ports may not work properly. The Roboocyte2
supports two transfer ports.
The Gilson liquid handler is used for delivering compounds directly to the perfusion inlet of the
TEVC probe. The input flow is controlled by the Roboocyte2 via the Minipuls 3 peristaltic pump.
Multi Channel Systems MCS GmbH recommends to use a yellow tubing ID 1.42 mm (see also
"Sources of Supply") with the Gilson Minipuls 3 peristaltic pump and to use a pump speed of
25 rpm. The resulting flow rate should be in the range of 5 to 6 ml per minute. Considering a
volume of 200 μl in the well, the solution should be exchanged after about 2 s. Please refer also
to the Gilson Minipuls Manual, which describes the relationship between tubing ID and flow rate.
For information on automated operation of the liquid handler, please refer to the Java scripting
language manual. Please see the Minipuls 3 Peristaltic Pump User’s Guide and the GX-271 Liquid
Handlers User's Guide from Gilson if you have questions concerning the general operation of the
peristaltic pump or the liquid handler, respectively.
6.3.1 Using the Transfer Ports of the Gilson GX-271
Gilson GX-271 transfer port station
Please note that the information in this chapter "Using the Transfer Ports" applies to Gilson
model GX-271 only. Transfer ports can be used for aspirating solutions from large reservoirs or
bottles, for example, for often used solvents such as Ringer or a reference compound. The transfer
port lines are closed by solenoid valves when they are not in use, to avoid that either liquid drips
out or air is sucked into the transfer port lines, and to avoid salt crystallization inside the port.
(Please keep in mind to include the appropriate valve control commands in your protocols. See the
Roboocyte2 Scripting Language manual for more information.) The GX-271 rinse and transfer port
station combines two transfer ports with a drain and rinse station e.g. for emptying the tubing at
the end of the working day or for rinsing the needle. For information on the installation of the
rinse and transfer port station, please refer to chapter "Setting Up the Gilson GX-271 Liquid
Handler".