Multichannel Systems Roboocyte2 Manual - Manual (Page 81)

Recording with Roboocyte2 Control Software
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7.5 Recording Data
Manual Recording Mode
What You “Can Do”:
 moving the measuring head into wells
 measuring the resistance of the electrodes
 determining electrode voltage offsets and compensate them
 impaling oocytes, measure membrane potential, clamp either current or voltage
 changing and testing various gain settings
(P gain, I gain, crosstalk capacitance compensation (CX))
 switching on and off perfusion, switch between different solutions
 recording and saving the data in continuous recording mode at various sampling frequencies
 recording IV dependencies
What You “Can Not Do” (Roboocyte2 Version 1.3.1):
 executing scripts
 saving the recorded IV dependencies
 linking solution exchanges to saved data from continuous recordings
7.5.1 Manual Recording Step by Step
1. Move the carrier to the respective well, for example to well A1. This will only work if you have
loaded or created a well plate before.
2. Move the measuring head down into the liquid (wells should be filled with at least 200 μl
of buffer).
3. Check the resistance of both glass microelectrodes. Resistances of a new measuring head should
be clearly below 1 MOhm.