Multichannel Systems Roboocyte2 Manual - Manual (Page 79)

Recording with Roboocyte2 Control Software
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Click on the "ResistancePos" button to move the carrier to the "Resistance Check Position" and
the measuring down. In order to be able to perform a resistance check you have to place one of
the small plastic dishes on the resistance position metal ring and fill it with oocyte Ringer solution.
After moving the measuring head to into the resistance position dish, you can measure the
electrode resistance by a click on the "Measure" button. A fresh measuring head should have
resistances of about 400-700 kOhms.
Click on the "Finish" button to bring z-axis and well plate carrier to their home positions and you
are ready to start the recording either in “Manual Mode” or automated by running a script.
7.4 Liquid Handling
Preparing Solutions
Before starting an experiment you should prepare all solutions to be used and prime all tubings
of either Roboflow or Gilson Liquid Handler. You should do this after installing the measuring
head and after connecting the inflow and outflow tubing to the measuring head.
Roboflow
In order to prime the tubings, open in main menu "Tools" the "Liquid Handling" tab of the
"Manual Mode" window. Connect all 12 tubings via the magnetic valves to the 12-fold manifold
and place the tubes into the desired solutions. If you want to use less than 12 different solutions
in your experiments, place the tubings not used in buffer solution, because the solution exchange
systems works properly only when all tubes are filled with liquid.
To start the priming procedure, be sure that all tube endings are placed in the respective
solutions. Either put a small dish underneath the measuring head or use one of the small dishes
either on "Park" or "Resistance" Position. Please use the respective movement commands in the
"Movement" tab of the "Manual Mode". Select 5000 for speed of valve an waste pump and 20 s
for the "Rinse Time". Then press "Start" and all 12 valves will be opened sequentially for 20 s
each. If there are still air bubbles in one or several tubings, repeat the rinse procedure until all
air bubbles have gone. The settings about 1.7 ml for every solution will be used for each rinse run.