Multichannel Systems Roboocyte2 Manual - Manual (Page 85)

Recording with Roboocyte2 Control Software
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In addition, you can execute IV protocols to work with voltage gated ion channels or test the
voltage dependence of ligand gated channels or transporters. For details about how to define
IV protocols please refer to the respective chapter of this manual. The examples below show
IV recordings from an oocyte expressing P2X2 receptors in presence and absence of 30 μM ATP.
Please note the different scaling of the current axis.
7.5.2 Automated Recording Step by Step
In the previous chapter the manual recording abilities of the Roboocyte2 have been presented.
All these single "manual" steps can be performed automatically for one cell after the other by
using a recording script. To start an automated recording you have to prepare the Roboocyte2
and the software as follows.
1. Place the oocytes into the well plate or use a well plate with injected cells.
2. Put the well plate onto the carrier.
3. Prepare and install the measuring head.
4. Prepare the solutions and prime the tubings in order to get rid of any air bubbles.
5. Define or select a liquid configuration that meets exactly the liquids used.
6. Make an alignment of the measuring head to the alignment tool.
7. Load the right script.
8. Select the oocytes to be measured.
9. Start the script recording.
Please refer to the Scripting manual for details about how to write a script.