Ocean Optics Curie UV-VIS Emission - Manual (Page 55)

C: Relative Irradiance Mode
Note
If you are using Microsoft Excel, choose Tools | Add-Ins and select AnalysisToolPak
and AnalysisToolPak-VBA.
Calibrating the Spectrometer
► Procedure
Perform the steps below to calibrate for relative irradiance:
1. Disable the light source in the Curie system by using the power switch on the light source or by
disabling the spectrometer strobe function on the Strobe tab in the Configure Data Acquisition
screen in the OOIBase32 software.
2. Connect the light source to the cuvette adapter via an optical fiber, and then insert the adapter into
the cuvette holder of the Curie system so that light from the lamp enters the spectrometer.
3. Place OOIBase32 into Scope mode by clicking the Scope Mode icon ( ) on the toolbar, or by
selecting Spectrum | Scope Mode from the menu bar, or by typing CTRL + SHIFT + S.
4. Take a reference spectrum of your light source. Adjust the integration time (or the A/D
conversion frequency) until the spectrum is on the scale (below 4000 counts).
5. In the Reference Color Temperature dialog box, enter the color temperature of the light source
(in Kelvin) and click OK. For the LS-1-LL, enter 2800.
6. Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference
from the menu bar to store the reference. This command merely stores a reference spectrum in
memory. You must select File | Save | Reference from the menu bar to permanently save the
spectrum to disk.
7. Turn off the LS-1-LL light source. Then, take a dark spectrum by clicking the Store Dark
Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This
command merely stores a dark spectrum in memory. You must select File | Save | Dark from the
menu to permanently save the spectrum to disk.
8. Place OOIBase32 into Relative Irradiance mode by clicking the Relative Irradiance Mode icon
( ) on the toolbar, or by selecting Spectrum | Relative Irradiance Mode from the menu bar.
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