Ocean Optics Curie UV-VIS Emission - Manual (Page 48)

B: Calibrating the Wavelength of the Curie Spectrometer
Calibrating the Wavelength of the Spectrometer
Preparing for Calibration
To recalibrate the wavelength of your Curie spectrometer, you need the following:
‰
A light source capable of producing spectral lines.
Note
Ocean Optics’ HG-1 Mercury Argon Calibration Light Source is ideal for recalibration. If
you do not have an HG-1, you will need a light source that produces several (at least 4-6)
spectral lines in the wavelength region of your spectrometer.
‰
The Curie system
‰
An optical fiber (maximum 50 µ m works best)
‰
PS-HG1-ADP Cuvette Adapter
‰
A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-
order linear regressions
Note
If you are using Microsoft Excel, choose Tools | Add-Ins and select AnalysisToolPak
and AnalysisTookPak-VBA.
Calibrating the Spectrometer
► Procedure
Perform the steps below to calibrate the wavelength of the Curie spectrometer:
1. Connect the HG-1 to the cuvette adapter via an optical fiber, and then insert the adapter into the
cuvette holder of the Curie system so that light from the lamp enters the spectrometer.
2. Place OOIBase32 into Scope mode and take a spectrum of your light source. Adjust the
integration time (or the A/D conversion frequency) until there are several peaks on the screen that
are not off-scale.
3. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.
4. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.
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